With reference to the text series of Talpatepiliyam, it is mentioned that leaves and stem bark of Ankenda Acronychia pedunculata are used externally in all types of oduvana and gadu. Staphylococcus aureus is the common cause of skin infections such as abscesses and wounds. This study was aimed to investigate whether there is antibacterial activity of fresh extracts of stem bark, leaves and combined sample stem bark and leaves of Acronychia pedunculata grown in Sri Lanka againstS. aureus ATCC25923 and Minimum Inhibitory Concentration MIC was studied. Kirby Baurer Well diffusion method of Antibacterial Susceptibility Testing was used to determine antibacterial activity of each fresh extract. Amoxicillin 10mg ml and distilled water were used as positive and negative control respectively. Zone diameters were interpreted and data were statistically analyzed. The results showed that there was antibacterial effect of each sample. The highest zone diameter was observed for the combined sample inhibition zone 11.67mm . This could be due to the synergetic activity of each compounds from each plant material used. Fresh extracts of stem bark showed the second most zone diameter 11mm while fresh extracts of leaves the least 9mm . As fresh extracts of combined sample showed the highest activity, dilution series for MIC were prepared from the combined sample. The MIC of combined sample for the growth of S. aureus was 10 2 mg ml. This study could be further investigated for more gram positive and negative bacterial and fungal species. Charitha. L. Muthukuda | Tilindra. R. Jayakody "Antibacterial Effect of Acronychia Pedunculata Fresh Extract against Staphylococcus Aureus: A Study in Vitro" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-4 , June 2021, URL: https://www.ijtsrd.compapers/ijtsrd43735.pdf Paper URL: https://www.ijtsrd.commedicine/ayurvedic/43735/antibacterial-effect-of-acronychia-pedunculata-fresh-extract-against-staphylococcus-aureus-a-study-in-vitro/charitha-l-muthukuda

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